The Anorectic Actions of the TGFβ Cytokine MIC-1/GDF15 Require an Intact Brainstem Area Postrema and Nucleus of the Solitary Tract
Figure 3
MIC-1/GDF15 activates both TH-positive and TH-negative neurons.
Photomicrographs of coronal sections through the rostro-caudal span of NTS, demonstrate the distribution of dual Fos (Panel A, green), and TH immunoreactivity (Panel B, red). The merged images are in panel C, scale bar: 40 µm. (D) The Line graph shows the number of neurons that are Fos-positive or Fos and TH-positive in various AP/mNTS regions, identified as mm distance relative to the interaural line. (E-H) The bar graphs show the number of neurons that are immunoreactive to TH, or Fos, or both TH and Fos in mNTS, AP or DMX. Data are presented as mean ± sem. Abbreviations: DMX, dorsal motor nucleus of vagus; ap, area postrema; sp, subpostrema; cc, central canal; NTS, nucleus of the solitary tract; mNTS, medial region of nucleus of the solitary tract.