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Reusable and Mediator-Free Cholesterol Biosensor Based on Cholesterol Oxidase Immobilized onto TGA-SAM Modified Smart Bio-Chips

Figure 4

(A) CVs of 5.0 mM K3Fe(CN)6/PBS (0.1 M) for bare (black curve) and TGA-SAM (blue curve) modified electrodes; (B) CVs recorded in 0.1 M phosphate buffer solution of bare chip (black curve), ChOx modified chip (blue curve), and in presence of 0.1 mM cholesterol (green curve) solution; (C) pH effect of Au/TGA/ChOx electrode in 0.1 mM cholesterol solution with the bio-chip.

Scan rate: 0.1 V/s, RE: Ag/AgCl (saturated KCl), Supporting electrolytes: 0.1 M phosphate buffer solution.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0100327.g004