Loss of HMG-CoA Reductase in C. elegans Causes Defects in Protein Prenylation and Muscle Mitochondria
Figure 4
Exogenous mevalonate is essential for prenylation in the hmgr-1(tm4368) mutant.
The prenylation reporter pGLO-1P::GFP-CAAX becomes diffusedly distributed in wild-type L1 worms exposed to 0.5 mM fluvastatins for 24 hours (A–B) or in hmgr-1(4368) mutants deprived of mevalonate for 24 or 48 hours (C–E). hmgr-1(4368) L1s deprived of mevalonate for 24 hours then provided with mevalonate for 24 hours show a clear restoration of prenylation (F). (G) Quantification of the degree of prenylation in the different genotypes and treatments. ***p<0.001 using Student’s t-test. Arrowheads indicate membrane enriched GFP.