B-Cell Epitopes in GroEL of Francisella tularensis
Figure 10
X-ray crystallographic structures of the antigen-binding sites of Ab53 and Ab64.
(A) Head-on views of the molecular surfaces, colored gray for the H chain and purple for the L chain, with the CDR loops colored and indicated as H1, H2, H3 for the H chain and L1, L2, L3 for the L chain. (B) Ribbon diagrams of the binding-sites, colored gray for VH and purple for VL, and clipped/depth-cued for clarity. Selected side chains are shown in stick and labeled in black for VH residues and purple for VL residues. Solvent molecules present in the structures (sulfate, chloride, tris) are also shown. Hydrogen bonds indicated in the text are shown as dotted lines. (C) Ribbon diagrams of the binding-sites with the docked FtGroEL model, shown in red for Ab53 with the side-chain of R362 and in green for Ab64 with the side-chain of K344. The positions of some of the residues in the docked antibody structures are somewhat shifted compared with the crystal structures in B due to the energy minimization steps used during the docking protocol. Figure made with Maestro (version 9.3.5, Schrödinger, Inc., New York, NY).