B-Cell Epitopes in GroEL of Francisella tularensis
Figure 5
Crosscompetition between 14 GroEL mAbs reveals at least three non-overlapping epitope areas in FtGroEL.
(A) Competition ELISA. HRP-conjugated streptavidin was used to detect the binding of biotinylated reporter antibodies (indicated by *) to native GroEL (except for Ab12, which was detected by HRP-conjugated anti-mouse IgG3), in the presence of the indicated competitor mAbs. (B) Data summary. Competition, defined as a ≥80% increase in percent inhibition is indicated by +; a 40–79% increase in percent inhibition is indicated by ±; and ≤39% increase in percent inhibition is indicated by –. Four groups of mAbs based on similarity in crossblocking profiles are colored red, orange, green or blue. NA, not applicable; ND, not done.