ROP18 Is a Key Factor Responsible for Virulence Difference between Toxoplasma gondii and Neospora caninum
Figure 2
Identification of Nc1-TgROP18.
(A) Electrophoresis of the PCR products. Both the GFP and TgROP18 target genes (717 bp and 1,662 bp, respectively) were amplified from the genomic DNA of the Nc1-TgROP18 strain. (B) qRT-PCR comparison of TgROP18 expression in RH strain and Nc1-TgROP18 strain. Data are mean ± SD (error bars) of three independent experiments. (C) and (D) Expression of TgROP18 in the cloned parasites was confirmed by Western blot analysis. The TgROP18 and GFP fusion protein (∼87 kDa) was recognized by both anti-GFP and anti-TgROP18 polyclonal antibodies.