N-Terminal Truncated UCH-L1 Prevents Parkinson's Disease Associated Damage
Figure 2
Structural differences between UCH-L1 and NT-UCH-L1.
(A, B) Purified UCH-L1 and NT-UCH-L1 were subjected to HDX studies. UCH-L1 and NT-UCH-L1 were incubated with D2O exchange buffer at 25°C for the indicated times and analyzed using nanoAcquity™/ESI/MS. HDX spectra of UCH-L1 and NT-UCH-L1 and deuterium exchange rates were represented by % exchange (A) and mass increase (B). (C) Recombinant UCH-L1 and NT-UCH-L1 were subjected to HDX studies. Proteins were incubated with D2O exchange buffer at 25°C for 30 min, digested with trypsin and analyzed using nanoAcquity™/ESI/MS. Deuterium exchange rates were represented by % exchange and colored accordingly.