Dose-Dependent Effect of Estrogen Suppresses the Osteo-Adipogenic Transdifferentiation of Osteoblasts via Canonical Wnt Signaling Pathway
Figure 2
Effect of 17beta-estradiol on adipogenic markers of osteo-adipogenic transdifferentiation of MC3T3-E1 cells.
After 14 days’ osteogenesis, 17beta-estradiol accompanied with or without ICI was added in adipogenic medium for 14 days and 14 days’ osteogenesis was used as positive control. O14∶14 days’ osteogenesis; O14+O14∶28 days’ osteogenesis; O14+A7∶7 days’ adipogenesis after 14 days’ osteogenesis; O14+A14∶14 days’ adipogenesis after 14 days’ osteogenesis; O14+A14+E2∶14 days’ adipogenesis accompanied with 10−7 M of 17beta-estradiol after 14 days’ osteogenesis; O14+A14+E2+ICI: 14 days’ adipogenesis accompanied with 10−7 M of 17beta-estradiol and ICI after 14 days’ osteogenesis. A: Effect of 17beta-estradiol on the Oil red staining of osteo-adipogenic transdifferentiation of MC3T3-E1 cells. B: lipid quantification of each group was valued by the method mentioned in “method and material.” C: Effect of 17beta-estradiol on the adipogenic mRNA expression of PPARγ, Fabp4, Dlk1, Gata2 and Wnt10b. D: Effect of 17beta-estradiol on brown adipogenic mRNA expression of Myf5, Elovl3 and Cidea. Expression of each target gene was calculated as a relative expression to beta-actin and represented as normalized fold expression. Data are represented as mean±SD of 3 independent experiments. *P<0.05 and **P<0.01.