Cathelicidin Host Defence Peptide Augments Clearance of Pulmonary Pseudomonas aeruginosa Infection by Its Influence on Neutrophil Function In Vivo
Figure 2
Exogenous LL-37 promotes an early neutrophil response to P. aeruginosa.
Wild type C57Bl/6 mice were inoculated with 3×107 cfu of P. aeruginosa PAO1 or PBS and 10 µg LL-37 peptide or PBS by intranasal delivery. At 6 hours (a, b & e) or 24 hours (c, d & f) after inoculation mice were culled and their lungs were lavaged. BALF was cytocentrifuged and differential counts were conducted for neutrophils (a–d) and monocytes (e & f). Data show Tukey box and whiskers plots for infected (a, c, e & f) (n≥9 per condition) and uninfected (b & d) animals (n≥5 per condition). Analyses were conducted using the Mann Whitney test; * p<0.05. ND denotes “not detected”.