Sequence Variations of Full-Length Hepatitis B Virus Genomes in Chinese Patients with HBsAg-Negative Hepatitis B Infection
Figure 1
Rolling circle amplification (RCA) of full-length HBV genome.
The number of initial HBV DNA template present in each RCA reaction is shown at the top. M, molecular weight marker; H2O, negative control. (A) The high-molecular-weight raw RCA products containing multiple copies of the initial HBV template. (B) The full-length HBV genomes recovered by restriction enzyme, SpeI digestion. (C) Full-length HBV genome amplified by using the RCA products as PCR template.