Human Retinal Transmitochondrial Cybrids with J or H mtDNA Haplogroups Respond Differently to Ultraviolet Radiation: Implications for Retinal Diseases
Figure 1
Differential growth patterns were observed for H cybrids versus J cybrids in UV-treated and untreated groups.
Cybrids containing J haplogroup mtDNA showed a steeper slope for the growth curve than H haplogroup mtDNA in both UV-treated and untreated groups. In untreated there were constant increases in growth patterns for both H and J cybrids. In the UV-treated samples, the growth rates slowed or declined until approximately 96 hours post treatment, and then increased again. Triplicate wells were run for each sample and the experiments were repeated twice. During the growth curve assay, each sample was run in triplicate. Grey solid line, untreated H cybrids; Grey broken line, UV-treated H cybrids; Black solid line, untreated J cybrids; Black broken line, UV-treated J cybrids.