Neuroproteome Changes after Ischemia/Reperfusion Injury and Tissue Plasminogen Activator Administration in Rats: A Quantitative iTRAQ Proteomics Study
Figure 1
Schematic of 2D LC-MS/MS experimental design.
Five iTRAQ labels were used: 113 for the Sham+distilled water injection. 114 for the Sham+rt-PA infusion. 115 for tMCAo+distilled water injection. 116 for tMCAo+rt-PA infusion. 121 for a pooled sample containing equal amounts from each group. Treated rats were given 6 mg/kg rt-PA 4 hrs after occlusion. All samples were pooled after iTRAQ labeling and subjected to mass spectrometric analysis.