The dlx5a/dlx6a Genes Play Essential Roles in the Early Development of Zebrafish Median Fin and Pectoral Structures
Figure 2
Expression of dlx5a during zebrafish pectoral development.
Whole mount in situ hybridization for dlx5a in the pectoral region of zebrafish embryos from 24 hpf to 72 hpf. During pectoral fin formation, dlx5a is expressed in apical ectodermal cells of the presumptive pectoral fin bud (PPF) at 24 hpf (A) and in the apical ectodermal ridge (AER) of the early pectoral fin bud (PFB) at 36 hpf (B). At 48 hpf, dlx5a expression is detected in the pectoral fin fold (PFF) and weak expression is observed in endochondral cells (end) of the PFB. Moreover, the transcripts are detected in the developing cleithrum (cl) from 36 hpf to 72 hpf (B–D). The dashed lines in B–C indicate the limit between the AER/PFF structures and cleithrum precursor cells. Note the absence of dlx5a expression in the PFF at 72 hpf (D). Scale bars 50 µm.