F-Spondin Deficient Mice Have a High Bone Mass Phenotype
Figure 1
Targeting strategy for generation of Spon1−/− mice.
(A) The murine Spon1 gene was disrupted by homologous recombination to replace exon 1 (represented by grey box) with an IRES/bGeo/PolyA cassette. Numbered arrows (red) indicate PCR primers used for genotyping of WT and mutant loci. (B) Identification of Spon1 mutant mice by PCR. Wild type (WT) mice were distinguished by PCR products from primer set 3 + 4, null mice (−/−) were positive for 1 + GT, and heterozygous mice (+/−) were positive for both. (C) RT-PCR analysis of Spon1 expression in selected tissues. Gapdh amplification was performed as a housekeeping control.