CD47-Independent Effects Mediated by the TSP-Derived 4N1K Peptide
Figure 6
4N1K-mediated inhibition of adhesion occurs in a CD47-independent manner.
A) Jurkat, JinB8 and JinB8-CD47 cells were labeled with Celltracker Green, then treated with buffer, 50 µM 4N1K, or 1 mM MnCl2 for 20 min prior to seeding onto fibronectin-coated wells and % adhesion calculated as stated before. Data as plotted are the means ± standard deviation of three replicate samples of one experiment (*, p<0.05) that is representative of three independently conducted ones. B) Same as in A) but with titrating amounts of 4NGG and 4N1K treatment of cells.