Phytophthora sojae Effector PsCRN70 Suppresses Plant Defenses in Nicotiana benthamiana
Figure 5
Down-regulation of the defense-associated genes in N. benthamiana by PsCRN70.
A. Relative expression levels of the PR1b, PR2b, ERF1 and LOX genes. The total RNA was extracted from the leaf tissues that transiently expressing PsCRN70 and GFP, respectively, and the expression levels of the indicated genes were measured using qRT-PCR. B. Relative expression levels of the indicated genes in the stable transgenic N. benthamiana. The N. benthamiana leaves 36 hpi with P. parasitica zoospores were collected, and the gene expression levels were measured by qRT-PCR. The gene expression levels were normalized to the EF1α gene. Bars represent the standard deviation (SD), with significant difference (** for P<0.01 and * for P<0.05, Student's t-test).