β2-Adrenoceptor Involved in Smoking-Induced Airway Mucus Hypersecretion through β-Arrestin-Dependent Signaling
Figure 4
Effect of β-arrestin knockdown on CSE-stimulated MUC5AC expression.
Cells were transfected with Lipofectamine2000 alone (vehicle), a nonspecific control siRNA (NC-siRNA, 100 nM), β-arrestin1–targeted siRNA (A1-siRNA, 100 nM) or β-arrestin2–targeted siRNA (A2-siRNA, 100 nM) for 48 h before incubation with or without CSE for 24 h. (A, B) Western blot analysis of β-arrestin1 and β-arrestin2 protein level to access the knockdown efficiency. (C, D) Quantitative RT-PCR analysis of MUC5AC mRNA expression. Data are means ± SEM (n = 3). NS, not significant.