New Aspects on the Structure of Neutrophil Extracellular Traps from Chronic Obstructive Pulmonary Disease and In Vitro Generation
Figure 3
Individual PMNs in various stages of NETosis.
COPD sputa immunolabelled for NE (green: A,B), citH3 (green: C–F), and PAD4 (red: F). DNA stained with either PI (red: A–D) or DAPI (blue: F). A: Non-activated stage with still lobulated nucleus, NE confined to cytoplasm. B: advanced stage with highly swollen nucleus, NE colocalised with chromatin (staining to the right extracellular). C–E: Stages during nuclear swelling. C: Nuclear lobulation still visible, part of chromatin stains for citH3. D: Staining for citH3 most intense in perinuclear cytoplasm. E: Chromatin extrusion. F: CitH3 and PAD4 colocalised in cytoplasm and nucleus. Scale bars are all at 5 µm and valid for each photograph within the individual combinations A–F.