Leishmania Eukaryotic Initiation Factor (LeIF) Inhibits Parasite Growth in Murine Macrophages
Figure 2
Effect of pre-infection treatment of J774A.1 macrophages with LieIF/IFN-γ on intracellular L. donovani growth inhibition.
(A) at early (4 h) and (B) late (72 h) time points after infection. J774A.1 cells were stimulated with recombinant LieIF (10 µg/ml) and recombinant ΙFN-γ (1 ng/ml). L. donovani growth inhibition at the late time point (72 h) is associated with reduction of the infection rate (C) and the parasite load (D). The parasite growth inhibition was determined by the colorimetric method alamarBlue and OD was measured at 570/630 nm. Infection rate and parasite load were determined by microscopic observation upon Giemsa's staining of slides to count the mean number of infected macrophages considering 200 macrophages and the mean number of intracellular amastigotes in 200 infected macrophages, respectively. Data are presented as the mean ± S.D of at least three independent experiments. Asterisks indicate statistically significant differences (p≤0.05).