Contribution of Intestinal Barrier Damage, Microbial Translocation and HIV-1 Infection Status to an Inflammaging Signature
Figure 5
Identifying a plasma signature of inflammaging.
The plasma concentrations of iFABP, sCD14, sCD27, hsCRP were incorporated into a k-means clustering analysis and linear discriminate function fit to the HIVneg donors. (A) WSS plot supporting a two cluster solution. (B) Age distribution of HIVneg subjects classified as IP+ (grey bar) or IP- (open bar). (C) The age distribution of IP+ (closed squares) or IP- (open squares) HIVpos subjects. For clinical parameters, the median (dot plots) or the event frequency (bar graph) in the HIVpos subjects is shown. In all cases the IP+ subjects are shown in closed squares or grey bars and compared to IP- subjects shown in open squares or open bars. Statistical significance was determined using the Mann-Whitney U test or Fisher's exact test as appropriate, p-value < 0.05 was significant. (D) CD4 T cell count at draw, (E) CD4+ T cell nadir, (F) Infection duration. (G) VACS index. (H) Plasma LPS concentration. To remain consistent with previous biomarker analysis: the plasma concentration was natural log transformed and a T-test was used to determine statistical significance. The horizontal line indicates the geometric mean. *-p = 0.04 (I) CMV IgG titer in the plasma. (J) Frequency of HIV/HCV co-infection. Subjects that had a record of detectable hepatitis C (HCV) nucleic acid (DNA or RNA) and/or a positive antibody test were considered HCV co-infected. If no HCV testing was noted subjects were excluded.