Intermediate-Type Vancomycin Resistance (VISA) in Genetically-Distinct Staphylococcus aureus Isolates Is Linked to Specific, Reversible Metabolic Alterations
Figure 1
Principal component analyses of the metabolic profiles of the SG series (A) and the JH series (B) show clustering of replicates by resistance phenotype.
(A) Principal component analysis of the metabolic profiles of isolates from the SG series (SG-S, SG-R and SG-rev) shows a separation of samples (replicates) by resistance phenotype. Specifically, the metabolic profiles corresponding to replicates of the VISA strain, SG-R (red ellipse) cluster separately from those of its parent VSSA, SG-S (black ellipse), while those of SG-rev (green ellipse) cluster between these two, overlapping both. (B) Principal component analysis of the metabolic profiles of isolates from the JH series again shows clustering of replicates by phenotype, with both the parent VSSA JH1 (black circles) and VISA JH2 (red circles) forming non-overlapping clusters (though one outlier, JH2-10, is noted).