A DNA Sequence Recognition Loop on APOBEC3A Controls Substrate Specificity
Figure 1
rA3A produced in E. coli binds and deaminates ssDNA.
A) rA3A was visualized by Coomassie staining B) The extent of deamination was determined for rA3A incubated with ssDNA for 2, 5, 10, 20, 40, or 60 minutes. C) DNA binding was determined by measuring the change in the intrinsic fluorescence of rA3A following incubation with an increasing amount of ssDNA.