Optical Stimulation of Zebrafish Hair Cells Expressing Channelrhodopsin-2
Figure 3
Mechanical stimulation is required for the temporal fidelity of phase-locked spiking.
(A) Top: Phase-locked spiking for a mechanical 20-Hz stimulus. Middle: Phase-locked spiking for an optical 20-Hz stimulus. Bottom: 20-Hz stimulus protocol (scale bar 25 ms). Note that stimuli were 25-ms in length and delivered at a 20-Hz rate. (B) Polar plots from 60 sweeps of mechanical (upper) and optical (lower) stimulation. Plots constructed from all spikes elicited by 20-Hz stimulation of the cell shown in A. (C) The vector strength of phase-locked spiking for multiple cells recorded during 60 sweeps of 10, 20, and 40 Hz mechanical and optical stimulation. The bars represent the mean of the vector strength from all cells.