The Effect of Substrate Elasticity and Actomyosin Contractility on Different Forms of Endocytosis
Figure 2
Inhibition of actomyosin contractility has a major effect on cell morphology and F-actin cytoskeleton but only a moderate, time-dependent influence on Tf and CTb association.
(A) Epifluorescence microscopy images of REF52 cells 6 hours after seeding on FN-coated glass and stained for plasma membrane (WGA) and F-actin cytoskeleton (Phalloidin-TRITC). Cells were treated for 1 hour with 10 µM Y27632 or 50 µM blebbistatin, or left untreated as controls. Scale bars: 10 µm. Normalized MFI values for Tf (B) or CTb (C) association in presence or absence of 10 µΜ Y27632 or Blebbistatin, after a 10-minute or 1-hour incubation. Dot plots and mean values are presented. Normalized FSC (D) and SSC (E) intensity values of REF52 cells in presence or absence of 10 µΜ Y27632 or Blebbistatin as determined by flow cytometry analysis. Dot plots and mean values are presented with each dot representing an independent experiment.