Assessment of the Sensitizing Potential of Processed Peanut Proteins in Brown Norway Rats: Roasting Does Not Enhance Allergenicity
Figure 5
Allergen-specific degranulation of RBL cells sensitized with sera from i.p. study with Ara h 1.
RBL cells were passively sensitized with serum pools (diluted 1∶2) from groups immunized i.p. three times with 200 µg of NAra h 1 (open circles), HAra h 1 (grey triangles) or GAra h 1 (black squares). For degranulation cells were stimulated with dilutions of native or processed Ara h 1 (A) or extracts of peanut-water mixtures (B). Data are presented as percentage β–hexosaminidase release of total biological release induced by stimulation with 125 ng/well of anti-rat IgE. Symbols represent mean values ± SD for each serum pool. RBL: rat basophilic leukemia, Imm: immunization, PE: peanut extract, PB: peanut butter.