Opposing Effects of PI3K/Akt and Smad-Dependent Signaling Pathways in NAG-1-Induced Glioblastoma Cell Apoptosis
Figure 1
NAG-1-induced apoptosis in a restricted set of glioblastoma cell lines.
U87 MG, U118 MG, U251 MG, A172, LN-229, and T98G glioblastoma cell lines were infected by Ad-NAG-1 and Ad-Con at 50 MOI (multiplicity of infection), respectively. A, 24 h after infection, NAG-1 overexpression in cell lysates was verified by western blot. B, 24 h after infection, secreted NAG-1 in culture media was determined by ELISA. C, 48 h after infection, cell apoptosis was analyzed by FCM. 1, Ad-Con, 2, Ad-NAG-1. *, P<0.05, **, P<0.01 versus Ad-Con.