Anti-LRP/LR Specific Antibody IgG1-iS18 Impedes Adhesion and Invasion of Liver Cancer Cells
Figure 3
Quantification of cell surface LRP/LR levels on liver cancer (HUH-7) and leukaemia (K562) cells by flow cytometry.
Cells were labelled with primary antibody IgG1-iS18 (1∶25) and anti-human phycoerythrin (PE) secondary antibody. 20000 cells were analyzed across all three cell lines, and the median fluorescence intensities (MFI) were used as an indicator of cell surface LRP/LR levels. The MFI values indicated in the last column of Table 1 were used in order to construct this figure and it is noteworthy that the MFI value corresponding to the MCF-7 cell line was set to 100%. Experiments were performed in triplicate and repeated at least three times. **p = 0.0025, ***p<0.0001.