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Identification of Novel Regulatory Genes in Development of the Avian Reproductive Tracts

Figure 11

Cell-specific localization of mRNA and protein for ZEB1 in female reproductive tracts during their development.

Cell-specific expression of ZEB1 mRNA and protein in development of the female reproductive tract was demonstrated by in situ hybridization (A) and immunofluorescence analyses (B). Cell nuclei were stained with DAPI (blue). Legend: C, cortex; F, follicle; L, lacunae; LE, luminal epithelium; M, medullar; Ms, mesonephros; S, stroma. Scale bar represents 100 µm and 20 µm for first and second horizontal panels of (A) and 50 µm for (B). See Materials and Methods for a complete description of the methods.

Figure 11

doi: https://doi.org/10.1371/journal.pone.0096175.g011