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Yeast Frataxin Is Stabilized by Low Salt Concentrations: Cold Denaturation Disentangles Ionic Strength Effects from Specific Interactions

Figure 2

Thermal denaturation of Yfh1 at comparable anion and cation concentrations.

Thermal denaturation curves of 10 µM Yfh1 in a 10 mM HEPES buffer at pH 7.5, measured by monitoring the CD intensity at 220 nm as a function of temperature in the temperature range 0°C to 80°C in the presence of A) 2 mM NaF, NaCl, NaH₂PO₄ and Na₂SO₄ B) 2 mM NaCl, KCl, MgCl₂ and CaCl₂, C) 25 and 50 µM FeSO₄.

doi: https://doi.org/10.1371/journal.pone.0095801.g002