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Cloning and Characterization of the Human Trefoil Factor 3 Gene Promoter

Figure 5

Effect of Sp1 overexpression on transcription from the hTFF3 promoter.

Both pGL3-300 and a Sp1 over-expression plasmid were co-transfected into LS-174T cells and cultured for 48 h. A. Relative luciferase activity was measured and calculated. Data are presented as the mean ± SD (*P<0.05 vs. PCDNA3.1). B and C. Detection of hTFF3 protein by western blotting (*P<0.05 vs. PCDNA3.1).

Figure 5

doi: https://doi.org/10.1371/journal.pone.0095562.g005