Transcriptome Profiling and Physiological Studies Reveal a Major Role for Aromatic Amino Acids in Mercury Stress Tolerance in Rice Seedlings
Figure 3
Calcium accumulation and response of mitogen-activated protein kinase (MAPK) activity to Hg by dose and time in rice roots.
(A) Root samples were labeled with 10 µM Oregon Green 488 BAPTA-1, a calcium indicator, for 30 min, then treated with 25 µM Hg for various times (1∼24 h). Green fluorescence indicates the presence of calcium. Five control and 5 treated roots showed similar results. Magnification for all images was ×100. Rice roots were treated with (B) 25 µM Hg for various times. A 15-µg aliquot of crude protein was separated by SDS-PAGE and analysed by in-gel kinase activity assay and western blot with anti-phospho-extracellular signal-regulated kinase (ERK) or anti-ERK antibodies. Arrows indicate kinase-active bands.