Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog
Figure 5
NR4A1 protein expression in normal, vehicle- and CI-treated LV.
Upper panels show western blot and quantitation of NR4A1 in the I/R LV. The increase of NR4A1 protein in the I/R region is significantly attenuated by CI pretreatment. Lower panel a demonstrates very little NR4A1 (red) in the non-I/R LV region, while panel b demonstrates increased nuclear NR4A1 and its cytoplasmic migration in vehicle-treated I/R heart, but not in the CI-treated heart (panel c). Panel c also demonstrates the negative staining for NR4A1 in the endothelial cell nucleus (open arrow) that is located beside a red blood cell (white arrow). The yellowish color of red blood cell is caused by auto-fluorescence. Normal dog, n = 3; Veh dog, n = 6; CI dog, n = 5.