Trib3 Is Developmentally and Nutritionally Regulated in the Brain but Is Dispensable for Spatial Memory, Fear Conditioning and Sensing of Amino Acid-Imbalanced Diet
Figure 3
Long-term spatial memory and reversal learning ability in the Morris water maze is not altered in Trib3-deficient mice.
Data are means ± SEM from littermate Trib3−/− (n = 16) and Trib3+/+ (n = 10) mice. (A) Escape latencies during four days of hidden-platform training performed at four trials per day. (B) Pool quadrant occupancy in a probe trial performed 24 h after the completion of training. The submerged platform was removed from the pool and the swim trajectory of mice was monitored for 1 min. The pool quadrant that previously contained the platform is designated the target quadrant, and the time spent in each quadrant of the pool is presented as percent of the total search time. (C) Reversal training escape latencies during two days of training performed at four trials per day. For reversal training, the hidden platform was repositioned to the pool quadrant opposite of the initial platform location. (D) Results of a probe trial performed 24 h after reversal training. The probe trial was carried out as in B. The reversal target denotes the pool quadrant that contained the platform during the reversal trainings trials. (E) Swimming speed at different stages of the experiment. The mean speed ± SEM from 60-second swimming sessions with no platform are shown. The habituation session was performed one day before the start of training. For Trib3+/+, n = 5 per sex, and for Trib3−/−, n = 8 per sex.