ZFP580, a Novel Zinc-Finger Transcription Factor, Is Involved in Cardioprotection of Intermittent High-Altitude Hypoxia against Myocardial Ischemia-Reperfusion Injury
Figure 5
Association of ZFP580 up-regulation with ERK1/2 activation induced by I/R.
(A) ERK1/2 phosphorylation levels in hearts subjected to I/R. (B) ERK1/2 phosphorylation levels in H9c2 cells subjected to SI/R. Both IHA hypoxia (C) and HPC (D) increased ERK1/2 activation in the myocardium and in H9c2 myocardial cells subjected to I/R. Western blot results are expressed as the ratio of phosphorylated ERK1/2 relative to total ERK1/2. (E) Treatment with PD98059, a selective inhibitor of ERK1/2 phosphorylation, significantly suppressed SI/R-induced ZFP580 expression in H9c2 myocardial cells. A total of 6–8 rats were used per group for in vivo experiments, and in vitro experiments were repeated three times. Values are presented as mean ± SEM. *p<0.05 vs. control, #p<0.05 vs. I/R group.