Inhibition of mTOR-Dependent Autophagy Sensitizes Leukemic Cells to Cytarabine-Induced Apoptotic Death
Figure 5
Cytarabine inhibits mTOR and modulates AMPK/Akt/ERK signaling in HL-60 and primary leukemic cells.
HL-60 (A), PBMC from CML patients (B) or healthy controls (C) were incubated for the indicated time periods with cytarabine (3.2 μM) and the levels of phosphorylated/total mTOR, p70S6K, AMPK, Akt and ERK were determined by immunoblotting. The representative blots from three independent experiments (A) or three different PBMC samples (B, C) are shown, while the densitometry data are mean ± SD values (*p<0.05 compared to untreated cells).