Vasoreparative Dysfunction of CD34+ Cells in Diabetic Individuals Involves Hypoxic Desensitization and Impaired Autocrine/Paracrine Mechanisms
Figure 3
Surface expression of CXCR4, VEGFR1, and VEGFR2 in nondiabetic and diabetic CD34+ cells in hypoxic conditions (pO2 = 5 mm Hg) for 4 hours, expressed as percent change compared to the expression at zero time under standard incubator conditions (immediately prior to placing cells into a regulated oxygen environment).
Physiologic concentrations of oxygen for the inner retina, pO2 = 40 mmHg and hypoxia, pO2 = 5 mmHg were achieved using oxygen controlled glove boxes attached via a central airlock with O2, CO2, N2, temperature and humidity control. A, Expression of CXCR4 was lower in diabetic cells following hypoxia compared to nondiabetic cells (P<0.05, n = 10). B, Expression of VEGFR1 was increased in diabetic cells following hypoxia compared to nondiabetic cells (P<0.05, n = 10). C, Expression of VEGFR2 was decreased in diabetic cells under hypoxic conditions compared to nondiabetic cells (P<0.05, n = 10).