Polymerase I and Transcript Release Factor Acts As an Essential Modulator of Glioblastoma Chemoresistance
Figure 5
Establishment of the stablely transfected cells and the expression of PTRF in stable transfected cells.
(A) Morphology of U251AR cells transfected with shPTRF (U251ARshPTRF cells); (B) Morphology of U251 cells transfected with shPTRF (U251shPTRF cells); Light microscopy, 20× (A, B); Fluorescence microscopy, 20× (A, B); (C, D) The protein and mRNA expression of PTRF, caveoin1, and P-gp in U251AR cells after transfection with shPTRF and negative vector (shNC) by Western bolt and quantitative RT-PCR. (E, F) The protein and mRNA expression of PTRF, caveoin1, and P-gp in U251 cells after transfection with shPTRF and shNC as indicated by Western blot analysis and quantitative RT-PCR.