Whole-Genome Analysis of Temporal Gene Expression during Early Transdifferentiation of Human Lung Alveolar Epithelial Type 2 Cells In Vitro
Figure 3
Validation of a subset of candidate genes by qRT-PCR.
RNA was harvested from hAT2 cells cultured on collagen or Matrigel at 12 = 2. The relative expressions of SPOCK2, PLEKHO1, SPRED1, RAB11FIP1, PTRF/CAVIN-1, and RAP1GAP were assessed by TaqMan qRT-PCR and normalized to GAPDH and TBP. The fold-changes in relative expression over time are shown as graphs. The smaller, inset graphs show the expression results obtained from the Illumina BeadChip analysis, for comparison.