Activation of Platelet-Derived Growth Factor Receptor Alpha Contributes to Liver Fibrosis
Figure 5
PDGFRα-positive cells co-localize with PDGFRβ-positive cells in chronic CCl4 injured liver.
PdgfrαWT/nGFP mice were injected with CCl4 twice weekly for six weeks. PDGFRα-expressing cells are identified by nuclear-localized GFP (green). PDGFRβ-expressing cells are identified by IF (PDGFRβ; magenta). A) PDGFRα-positive cells are aligned between portal veins (PV). B) PDGFRβ is expressed in the same periportal area as PDGFRα-positive cells, as shown in the merged image (C). A–C) Scale bars are 100 μm. D–F) Higher magnification shows that PDGFRα and PDGFRβ co-localize in the same cell, based upon co-localization of the GFP and PDGFRβ signal. Scale bars are 10 μm.