Group I PAK Inhibitor IPA-3 Induces Cell Death and Affects Cell Adhesivity to Fibronectin in Human Hematopoietic Cells
Figure 4
IPA-3-induced decrease in cell adhesivity to fibronectin.
Top: Cells were treated for 2 h with 20 μM IPA-3 prior to 1 h incubation on fibronectin-coated surface. The fraction of adhered cells is shown as mean and standard deviation from independent experiments for each cell line (N = 3 to 5) as well as for peripheral blood mononuclear cells (PBMC) obtained from healthy donors (N = 3) or from leukapheresis (N = 2). White bars: untreated controls, grey bars: 20 μM IPA-3. The differences between treated samples and controls were statistically significant (*P<0.05, **P<0.01) with the exception of K562 cells. Lower graphs: Dose dependency of IPA-3-induced loss of cell adhesivity to fibronectin. ACF - adherent cell fraction.