The Ectodomain of TLR3 Receptor Is Required for Its Plasma Membrane Translocation
Figure 5
Localization of chimeric receptors with exchanged cytosolic domains of TLR3 and TLR9.
HEK293T cells were transiently transfected TLR3-3-9-YFP (A and B - yellow) or TLR9-9-3-YFP (C and D - yellow) and with UNC93B1. Plasma membrane markers SynaptoRed and CTB-Alexa 555 are shown in magenta. (A) Localization of TLR3-3-9-YFP on plasma membrane in cells overexpressing UNC93B1. (B) Intracellular localization of TLR3-3-9-YFP in HEK293T without overexpression of UNC93B1. (C) Intracellular localization of TLR9-9-3-YFP in cells overexpressing UNC93B1. (D) Intracellular localization of TLR9-9-3-YFP in HEK293T without overexpression of UNC93B1. (A–D) Data are representative of three experiments. TLR membrane localization was evaluated from plots (bottom) of normalized fluorescence intensities of TLR and plasma membrane (PM) within 3 μm line profiles (n = 9). Three representative lines are marked on merged images. Images are selected from three independent experiments. Scale bars, 10 μm.