Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells
Figure 1
Cytotoxic effects of combined SAHA and TRAIL treatment in the cell lines ESS-1 and MES-SA.
Cell viability (MTS) assays were performed after treatment of ESS-1 and MES-SA endometrial stroma sarcoma cells as well as HESC control cells for 24 hours with 3 μM of the HDAC inhibitor SAHA and/or different concentrations of human recombinant TRAIL (5, 25, 50, and 100 ng/ml) to determine the most effective cytotoxic concentration (A). Cell viability of ESS-1, MES-SA, and HESC control cells was determined in a time course experiment after treatment of cells for 0, 12, 24, 36, or 48 hours with 3 μM SAHA and/or 100 ng/ml TRAIL (B). The results in (A) and (B) are expressed as percentage of relative viability as compared to the untreated control. Cells were seeded at a density of 5×103 cells per well. Each value represents the average of 3 independent experiments with 5 replicates each. Asterisks (* p<0.05) or number signs (# p<0.001) indicate statistically significant differences as compared to the untreated control.