Extracellular Vesicles in Luminal Fluid of the Ovine Uterus
Figure 1
Transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA) of vesicles isolated from uterine luminal fluid of sheep.
(A–B) TEM revealed vesicles of about 100 nm, consistent with exosomes. (C) NTA profile of extracellular vesicle collection from one individual. Measurements were collected from 3 samples; error bars represent +/−1 standard deviation. The profile confirms size estimates from TEM images. (D) Nanoparticle Tracking Analysis of collected extracellular vesicles. Precipitated extracellular vesicles were suspended in PBS+0.1%BSA and analyzed by NTA using a NanoSight instrument. Mean diameter, standard deviation of the mean, and mode size are reported (Replicates ≥3 per sample). These measurements are consistent with the presence of exosomes and microvesicles in the collected samples from ULF.