Application of a New Dual Localization-Affinity Purification Tag Reveals Novel Aspects of Protein Kinase Biology in Aspergillus nidulans
Figure 10
CkiAHrr25 locates to mitotic SPBs and to septa independently of microtubules in benomyl treated cells.
A Time lapse images showing that CkiAHrr25-DLAP locates to the SPB/kinetochore region (arrowheads) independently of microtubules. Following transit through the mitotic state CkiAHrr25 locates to a forming septum (arrowhead 72′). B Time lapse images and kymographs from Video S3 showing a non-SPB CkiAHrr25 focus (arrows) which appears during the SAC arrest and persists into interphase. C A nucleus during SAC arrest in which an individual kinetochore (arrowhead) is detached from the SPB/kinetochore cluster. CkiAHrr25 is present at the SPB/kinetochore cluster but not at the unattached kinetochore. D A germling in the presence of benomyl with CkiAHrr25 locating to a mature septum (arrowhead). At 0′ the cells on either side of the septum are in interphase (i) and CkiAHrr25 is enriched in nuclei. CkiAHrr25 remains at the septum at 7′ when the cell on the right has entered a mitotic SAC arrest (M) indicated by CkiAHrr25 SPB location. Bar ∼10 µm.