Application of a New Dual Localization-Affinity Purification Tag Reveals Novel Aspects of Protein Kinase Biology in Aspergillus nidulans
Figure 7
The NimXCdk1 kinase in enriched in nuclei and at SPBs.
A NimXCdk1-DLAP concentrates to nuclei and SPBs during G2 but partially disperses from nuclei during mitotic entry. False coloring (NimXCdk1 *) more clearly reveals NimXCdk1 location to SPBs (arrowhead) and shows that NimXCdk1 accumulates in the cytoplasm as it is released from nuclei during mitosis. B and C Time lapse images and kymographs showing nuclei entering a mitotic SAC arrest in the presence of benomyl. NimXCdk1 remains concentrated around SPBs during SAC arrest. The arrowheads indicate an additional focus of NimXCdk1 present during SAC arrest which appears to be in the vicinity of the nuclear envelope. As shown in B, NimXCdk1 disperses during SIME prior to Nup49 reassembly at NPCs in the nuclear envelope. Bar ∼10 µm.