Candida albicans Uses the Surface Protein Gpm1 to Attach to Human Endothelial Cells and to Keratinocytes via the Adhesive Protein Vitronectin
Figure 2
The candida GPM1 deletion mutant bins with low intensity to human endothelial cells (HUVEC).
The candida gpm1Δ/Δ knock-out mutant bound with low intensity to HUVEC. Yeast cells of C. albicans SC5314 (wild type), gpm1Δ, gpm1Δ/Δ or gpm1Δ/Δ::GPM1 were labeled with DiD and incubated with DiO-labeled HUVEC for 120 min at 37°C at 5% CO2. After washing and detachment, HUVEC with associated (adherent and/or endocytosed) C. albicans cells were identified in flow cytometry as double-positive cells (DiO+, DiD+) and by the change in side scatter. HUVEC alone were detected as single-positive cells (DiO+, DiD−) were used as control.