Candida albicans Uses the Surface Protein Gpm1 to Attach to Human Endothelial Cells and to Keratinocytes via the Adhesive Protein Vitronectin
Figure 1
Candida albicans Gpm1 binds to human cells.
(A) Candida Gpm1 binds to HUVEC and HaCaT. Recombinant Gpm1 was added to human endothelial cells (HUVEC), keratinocytes (HaCaT) or monocytic U937 cells. After incubation and subsequent washing, bound Gpm1 was detected in flow cytometry using rabbit Gpm1 antiserum followed by Alexa Fluor 647 goat anti-rabbit IgG. Human cells without Gpm1 were used as control. Histograms are representative of three independent experiments. (B) Binding of Gpm1 to HUVEC and HaCaT was confirmed by confocal microscopy. HUVEC (top panels) or HaCaT cells (bottom panels) were incubated with Gpm1, fixed with parafolmaldehyde and after washing, bound Gpm1 was detected with rabbit Gpm1 antiserum followed by Alexa Fluor 488 goat anti-rabbit IgG (green). The cellular DNA was stained with DAPI (blue). Scale bar = 10 µm.