High Fat Diet Rapidly Suppresses B Lymphopoiesis by Disrupting the Supportive Capacity of the Bone Marrow Niche
Figure 3
High Fat Diet Initiates an Aged Leukocyte Phenotype in the Bone Marrow.
(a) A two-color flow cytometric method was used to isolate leukocyte populations of the lymphocyte (B and T-cells) and myeloid lineages, with the unstained control displayed in the left panel. (b–d) At two days a T-lymphopenia was present. At one week of HFD B-cell populations were diminished in the HF group, while T-cell populations were not significantly affected. After six weeks bone marrow lymphocyte populations were depressed and the myeloid lineage proliferated in response to HFD. The suppression of lymphocytes accompanied by myeloid expansion is a classic phenotype of hematopoietic aging. These changes may lead to defects in adaptive immunity. Each time point was drawn from distinct cohorts of animals, as such, comparisons can only be made within a time point due to variability in processing. *P<0.05 vs. RD.