CCR2 Gene Deletion and Pharmacologic Blockade Ameliorate a Severe Murine Experimental Autoimmune Neuritis Model of Guillain-Barré Syndrome
Figure 1
PCR verification of CCR2 genotype.
Representative digital images of ethidium bromide-stained agarose gels of resolved tail snip genomic DNA (A and B) and splenocyte cDNA (C and D) demonstrate mouse CCR2 genotype. Mice that lack CCR2 genomic DNA (A) and express the neomycin cassette (B) are knockout (KO), while wild type (WT) mice express CCR2 without the neomycin cassette. Heterozygote (HT) mice express both. KO mouse splenocytes do not express CCR2 cDNA, in contrast to HT and WT mice (C). GAPDH serves as an internal loading control and housekeeping gene for splenocyte cDNA loading (D).