Pretreatment of Epithelial Cells with Live Streptococcus pneumoniae Has No Detectable Effect on Influenza A Virus Replication In Vitro
Figure 1
Calibration of a standard curves to quantify different strains of S. pneumoniae.
Twelve different strains of S. pneumoniae were grown in THY medium and CFUs quantified based on OD value over the mid-exponential phase of growth. (A) A representative blood agar plate showing the number of CFUs at 16 hr of incubation in a series of 10-fold diluted S. pneumoniae broth culture in triplicate is shown. (B) Calibration curves were plotted for each strain to quantify 12 different S. pneumoniae strains in CFUs per mL over the OD600 values from 0.2 and 0.6. Each data point on the graph represents the average value of two experiments.